exercise and insulin

S A I N T L O U I S U N I V E R S I T Y

Jonathan Fisher, Associate Professor

joined SLU faculty in 2001

Insulin is a hormone that stimulates glucose (sugar) uptake

into muscle, heart, and fat tissue.

Muscle stores most of the glucose

that is transported out of the blood in response to insulin

Metabolic stress (e.g. exercise) increases insulin action in muscle.

Diabetes is often related to

resistance of muscle to insulin

and resultant abnormal elevation of blood glucose concentrations

We focus on factors that could potentially increase insulin-stimulated glucose transport into in skeletal muscle.

recent lab photo

Current/recent funding

R15 DK080437, 1/15/2008-12/31/2010, The role of ATM in glucose transport and insulin signaling in skeletal muscle.

3R15DK080437-01S2, 6/10/2009-12/31/2010, Supplement to provide summer research experiences for science teachers.

3R15DK080437-01S3, 1/1/2010-3/31/2010, Supplement to support purchase of a liquid scintillation counter (PerkinElmer TriCarb3110TR)

National Skeletal Muscle Research Center (NSMRC) Pilot Project, 9/1/2007-8/31/2008

K01 DK066330, 3/1/2004-12/31/07

Review articles

Lorraine P. Turcotte (Associate Professor, and Chair, Department of Kinesiology, University of Southern California) and Fisher JS. Skeletal muscle insulin resistance: roles of fatty acid metabolism and exercise, Physical Therapy, 88:1-18, 2008. [PDF] [PubMed]

Fisher JS. Potential role of the AMP-activated protein kinase in regulation of insulin action. Cellscience Reviews (online journal, ISSN 1742-8130), volume 2, issue 3.

Publications

*Jeong I, ^Patel AY, ^‡Zhang Z (Sigma-Aldrich), *Patil PB, ^Nadella ST, ^Nair S, ^‡Ralston L (Monsanto), ^†Hoormann JK, Fisher JS. Acta Physiologica In Press (accepted 11/23/2009). [PDF].

P. B. Patil*, S.D. Minteer⁺(SLU Chemistry), A.A. Mielke†, L.R. Lewis†, C.A. Casmaer^, E. J. Barrientos^, J. S. Ju*, J. L. Smith*, J. S. Fisher. Malonyl coenzyme A affects insulin-stimulated glucose transport in myotubes. Archives of Physiology and Biochemistry. 113:13-24, 2007. [PubMed] [PDF]

*Ju JS, *Gitcho MA, ^Casmaer CA, *Patil PB, ^Han D-G,⁺Spencer SA (SLU Biology), and Fisher JS. Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase. American Journal of Physiology: Cell Physiology, 292:C564-C572, 2007. [PDF] [PubMed]

Jill L. Smith*, Pankaj B. Patil*, Shelley D. Minteer+ (SLU Chemistry), Jason R. Lipsitz†, and Jonathan S. Fisher. Possibility of autocrine b-adrenergic signaling in C2C12 myotubes. Experimental Biology and Medicine 230:845-852, 2005. [PDF] [PubMed]

Jonathan S. Fisher, Jeong-Su Ju*, Peter J. Oppelt^, Jill L. Smith*, Atsushi Suzuki‡ , and Hiroyasu Esumi‡ (National Cancer Research Institute East, Japan). Muscle contractions, AICAR, and insulin cause phosphorylation of an AMPK-related kinase. American Journal of Physiology: Endocrinology and Metabolism 289:E986-E992, 2005. [PDF] [PubMed]

Marie C. Onan*, Jonathan S. Fisher, Jeong-Sun Ju*, Bryan C. Fuchs*, and Barrie P. Bode⁺ (SLU Biology). Type I diabetes affects skeletal muscle glutamine uptake in a fiber-specific manner. Experimental Biology and Medicine 230:606-611, 2005. [PDF] [PubMed] Link to Dr. Bode's page.

Jill L. Smith*, Pankaj B. Patil*, and Jonathan S. Fisher. AICAR and hyperosmotic stress increase insulin-stimulated glucose transport. J Appl Physiol 99:878-883. [PDF] [PubMed]

Jeong-Sun Ju*, Jill L. Smith*, Peter J. Oppelt^, and Jonathan S. Fisher. Creatine feeding increases GLUT4 expression in rat skeletal muscle. Am J Physiol Endocrinol Metab, 288:E347-E352, 2005. [PDF] [PubMed]

Jill L Smith*, Jeong-Sun Ju*, Bithika M Saha^, Brad A Racette‡ (Neurology, Washington University), and Jonathan S Fisher. Levodopa with carbidopa diminishes glycogen concentration, glycogen synthase activity, and insulin-stimulated glucose transport in rat skeletal muscle. J Appl Physiol 97:2339-2346, 2004. [PDF] [PubMed]

*graduate student

^ undergraduate student

†high school student

⁺collaboration with SLU faculty member

‡researchers from other institutions

more publications… (1997-2002)

ResearcherID link

Links:

Lab 232 Macelwane Hall [map]

Office 209 Macelwane Hall

fisherjs@SLU.edu

314-977-1712

New students in the lab will need:

1 Dept of Comparative Med Training/Orientation

2 chemical/biological safety training

3 radiation safety training

4 online bloodborne pathogen training

Training schedules and other lab safety information

Students who will use human samples will need

online HIPPA training

and online IRB training

Lab guidelines

Campus maps

map of Health Sciences Center area

Billiken Shuttle Service (van between health sciences campus and Busch Student Center)

Clay Semenkovich (WashU) describes a new molecular player in insulin resistance: ATM

Some really nice review articles on exercise-related insulin sensitivity have been written by:

John Holloszy [PDF] [Pubmed] and

Wojtaszewski, Nielsen, and Richter [PDF] [Pubmed]

Calbiochem simplified signaling pathways for Insulin/IGF-1 and mTOR

Recent profile of several Department of Biology faculty members in Universitas (starts on p. 8 of this pdf).

SLU Undergraduate Research Fund

Storm imaging procedures for western blots

http://www.ebi.ac.uk/clustalw/ multiple sequence alignment tool

enhanced Medline search http://www.biomedsearch.com/

ISI Web of Knowledge

Crisp database

Centers for Disease Control biosafety guidelines

list of useful buffers (Excel file) and recipes

European Bioinformatics Institute

calculate the net charge or isoelectric point of a peptide

lists of restriction enzymes

restriction enzyme site mapper with virtual digest

MIT primer3 primer selection program

rcf calculations for various rotors

motif scan in a protein sequence

prediction of mitochondrial targeting sequences

A boatload of domains at the SMART website.

Still more domains and motifs at the Prosite website

The Pfam Protein Families Database. Bateman A, Birney E, Cerruti L, Durbin R, Etwiller L, Eddy SR, Griffiths-Jones S, Howe KL, Marshall M, Sonnhammer EL (2002) Nucleic Acids Research. 30(1):276-280

Jörg Schultz, Richard R. Copley, Tobias Doerks, Chris P. Ponting and Peer Bork. SMART: a web-based tool for the study of genetically mobile domains. Nucleic Acids Research, 2000, Vol. 28, No. 1 231-234

How specific are your specific inhibitors?

Davies SP, Reddy H, Caivano M, Cohen P. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 2000 Oct 1;351(Pt 1):95-105.

http://www.biochemj.org/bj/351/0095/3510095.pdf

…and an update.

USDA Nutrient Data Laboratory page, containing food composition database (find out content of specific fatty acids, amino acids, etc.)

http://www.nal.usda.gov/fnic/foodcomp/

A new view of statistics. Everything you ever wanted to know about statistics.